Isolation and sequence of the gene encoding ornithine decarboxylase, SPE1, from Candida albicans by complementation of a spe1 delta strain of Saccharomyces cerevisiae

M D McNemar; J A Gorman; H R Buckley

doi:10.1002/(SICI)1097-0061(199711)13:14<1383::AID-YEA189>3.0.CO;2-M

Isolation and sequence of the gene encoding ornithine decarboxylase, SPE1, from Candida albicans by complementation of a spe1 delta strain of Saccharomyces cerevisiae

Yeast. 1997 Nov;13(14):1383-9. doi: 10.1002/(SICI)1097-0061(199711)13:14<1383::AID-YEA189>3.0.CO;2-M.

Authors

M D McNemar¹, J A Gorman, H R Buckley

Affiliation

¹ Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA.

PMID: 9392083
DOI: 10.1002/(SICI)1097-0061(199711)13:14<1383::AID-YEA189>3.0.CO;2-M

Abstract

The gene encoding ornithine decarboxylase, SPE1, from the pathogenic yeast Candida albicans has been isolated by complementation of an ornithine decarboxylase-negative (spe1 delta) strain of Saccharomyces cerevisiae. Four transformants, three of which contain plasmids with the SPE1 gene, were isolated by selection on polyamine-free medium. The C. albicans ornithine decarboxylase (ODC) showed high homology with other eukaryotic ODCs at both the amino acid and nucleic acid levels.

MeSH terms

Amino Acid Sequence
Base Sequence
Blotting, Southern
Candida albicans / enzymology*
Candida albicans / genetics*
DNA, Fungal / analysis
Genes, Fungal
Genetic Complementation Test*
Molecular Sequence Data
Mutation*
Ornithine Decarboxylase / genetics*
Ornithine Decarboxylase / isolation & purification
Phenotype
Saccharomyces cerevisiae / genetics*
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Transformation, Genetic

Substances

DNA, Fungal
Ornithine Decarboxylase

Associated data

GENBANK/U85005