Abstract
The strain SCSIO N160 was isolated from a South China Sea sediment sample and was characterized as a Micromonospora rosaria species on the basis of its 16S rRNA gene sequence. Three new fluostatins, I-K (1-3), were isolated from the culture of M. rosaria SCSIO N160, together with six known compounds, fluostatins C-F (4-7), rabelomycin (8), and phenanthroviridone (9). The structure of fluostatin D (5) was confirmed by an X-ray crystallographic study. The absolute configuration of 1 and 3 was assigned by electronic circular dichroism calculations. Compounds 8 and 9 exhibited good antimicrobial activities against Staphylococcus aureus ATCC 29213 with MIC values of 1.0 and 0.25 μg/mL, respectively. Compound 9 also exhibited significant in vitro cytotoxic activities toward SF-268 (IC50 0.09 μM) and MCF-7 (IC50 0.17 μM).
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Bacterial Agents / chemistry
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Anti-Bacterial Agents / isolation & purification*
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Anti-Bacterial Agents / pharmacology
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Antineoplastic Agents / chemistry
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Antineoplastic Agents / isolation & purification*
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Antineoplastic Agents / pharmacology
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Bacillus subtilis / drug effects
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Candida albicans / drug effects
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Crystallography, X-Ray
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Drug Screening Assays, Antitumor
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Escherichia coli / drug effects
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Female
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Fluorenes / chemistry
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Fluorenes / isolation & purification*
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Fluorenes / pharmacology
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Geologic Sediments
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Humans
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Microbial Sensitivity Tests
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Micromonospora / chemistry*
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Molecular Structure
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Nuclear Magnetic Resonance, Biomolecular
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Oceans and Seas
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RNA, Ribosomal, 16S / genetics
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Staphylococcus aureus / drug effects
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Streptomyces / chemistry
Substances
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Anti-Bacterial Agents
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Antineoplastic Agents
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Fluorenes
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RNA, Ribosomal, 16S
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fluostatin D
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fluostatin I
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fluostatin J
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fluostatin K