Defence cascade in Verticillium-infected grafted tomato

Plant Signal Behav. 2018;13(6):e1475807. doi: 10.1080/15592324.2018.1475807. Epub 2018 Jun 25.

Abstract

In tomato the Ve1-gene provides resistance to the vascular pathogen, Verticillium dahliae, race 1; ve1 plants are susceptible. Reciprocal grafts of resistant and susceptible tomato near-isolines were used to examine proteomic changes and, in particular, the effect of the Ve1-gene on the defence/stress protein cascade induced during Verticillium wilt. Based on label-free LC-MS, the results indicate that this defence response is cell-specific, correlates with overall fungal colonization and is mitigated by Ve1 function. The influence of the Ve1-gene in resistant tissues, however, is not actually transferred to susceptible tissues in the grafted plant.

Keywords: Ve R-locus; Ve1-gene; Verticillium wilt; defence genes; proteome; tomato.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Gene Expression Regulation, Plant
  • Plant Diseases / microbiology
  • Plant Proteins / genetics
  • Plant Proteins / metabolism
  • Proteomics / methods*
  • Solanum lycopersicum / metabolism*
  • Solanum lycopersicum / microbiology*
  • Verticillium / pathogenicity*

Substances

  • Plant Proteins

Grants and funding

This study was supported by NSERC (R.N.N. and J.R.) and NIH, NHLBI (A.K. and NSERC (R.N.N. and J.R.)); National Heart, Lung, and Blood Institute [N01-HV-00245]; Natural Sciences and Engineering Research Council of Canada [6768-2009 RGPIN]; Natural Sciences and Engineering Research Council of Canada [2961-2010 RGPIN];