Abstract
A biosynthetic gene cluster of the fungal xanthone heterodimer neosartorin (1) was discovered in Aspergillus novofumigatus, and its biosynthesis was investigated by a series of gene-deletion experiments. The results indicate that the two monomeric units of 1 are synthesized by the same set of enzymes, with chrysophanol (5) as a common precursor. Furthermore, the P450 monooxygenase NsrP for the heterodimerization was discovered, which also accepts non-native substrates to afford novel xanthone dimers.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Bacterial Agents / chemical synthesis*
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Anti-Bacterial Agents / chemistry
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Anti-Bacterial Agents / pharmacology
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Aspergillus / genetics*
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Aspergillus / metabolism
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Biological Products
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Biosynthetic Pathways / genetics
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Gene Deletion
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Genome, Fungal
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Methicillin-Resistant Staphylococcus aureus / drug effects
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Microbial Sensitivity Tests
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Molecular Structure
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Multigene Family*
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Xanthones / chemical synthesis*
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Xanthones / chemistry
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Xanthones / metabolism
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Xanthones / pharmacology
Substances
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Anti-Bacterial Agents
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Biological Products
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Xanthones