Directed evolution of biocatalysts

Curr Opin Chem Biol. 1999 Feb;3(1):54-9. doi: 10.1016/s1367-5931(99)80010-6.


Directed evolution is being used increasingly in academic and industrial laboratories to modify and improve important biocatalysts. Significant advances during this period of review include compartmentalization of genes and the in vitro translation apparatus in emulsions, as well as several impressive demonstrations of catalyst improvement. Shuffling of homologous genes offers a new way to utilize natural diversity in the evolution of novel catalysts.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Review

MeSH terms

  • Catalysis
  • Directed Molecular Evolution*
  • Enzymes / chemistry*
  • Enzymes / metabolism
  • Substrate Specificity


  • Enzymes