Structure and expression of variant BRCA2a lacking the transactivation domain

Oncol Rep. Mar-Apr 1999;6(2):437-40.

Abstract

BRCA1 and BRCA2 are tumor suppressor genes shown to be involved in 90% of familial breast cancers and also known to be involved in ovarian and prostate cancers. Both BRCA1 and BRCA2 gene products are regulated in a cell cycle-dependent manner and have potential transactivation function. Here, we show that BRCA2 undergoes differential splicing giving rise to a novel variant protein BRCA2a, lacking putative transcriptional activation domain. Both BRCA2a and BRCA2 are expressed at high levels in thymus and testis but moderate levels in mammary gland and prostate suggesting that BRCA2a and BRCA2 may have a role in the development and differentiation of these tissues.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alternative Splicing
  • Apoptosis Regulatory Proteins
  • BRCA2 Protein
  • Biomarkers, Tumor / genetics*
  • Breast / metabolism
  • Cloning, Molecular
  • Exons
  • Female
  • Genetic Variation*
  • Humans
  • Male
  • Neoplasm Proteins / genetics*
  • Organ Specificity
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Deletion*
  • Testis / metabolism
  • Thymus Gland / metabolism
  • Transcription Factors / genetics*
  • Transcriptional Activation

Substances

  • Apoptosis Regulatory Proteins
  • BLID protein, human
  • BRCA2 Protein
  • BRCA2 protein, human
  • Biomarkers, Tumor
  • Neoplasm Proteins
  • Transcription Factors