RFLAT-1: a new zinc finger transcription factor that activates RANTES gene expression in T lymphocytes

Immunity. 1999 Jan;10(1):93-103. doi: 10.1016/s1074-7613(00)80010-2.

Abstract

RANTES (Regulated upon Activation, Normal T cell Expressed and Secreted) is a chemoattractant cytokine (chemokine) important in the generation of inflammatory infiltrate and human immunodeficiency virus entry into immune cells. RANTES is expressed late (3-5 days) after activation in T lymphocytes. Using expression cloning, we identified the first "late" T lymphocyte associated transcription factor and named it "RANTES Factor of Late Activated T Lymphocytes-1" (RFLAT-1). RFLAT-1 is a novel, phosphorylated, zinc finger transcription factor that is expressed in T cells 3 days after activation, coincident with RANTES expression. While Rel proteins play the dominant role in RANTES gene expression in fibroblasts, RFLAT-1 is a strong transactivator for RANTES in T cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Binding Sites / genetics
  • COS Cells
  • Cell Cycle Proteins
  • Cell Line
  • Chemokine CCL5 / biosynthesis
  • Chemokine CCL5 / genetics*
  • DNA-Binding Proteins / biosynthesis
  • DNA-Binding Proteins / isolation & purification*
  • DNA-Binding Proteins / metabolism
  • DNA-Binding Proteins / physiology*
  • Gene Expression Regulation*
  • Humans
  • Kruppel-Like Transcription Factors
  • Lymphocyte Activation
  • Molecular Sequence Data
  • Organ Specificity / genetics
  • Promoter Regions, Genetic
  • Proto-Oncogene Proteins / metabolism
  • Proto-Oncogene Proteins c-rel
  • Repressor Proteins
  • T-Lymphocytes / immunology
  • T-Lymphocytes / metabolism*
  • Trans-Activators / physiology
  • Transcription Factors / biosynthesis
  • Transcription Factors / isolation & purification*
  • Transcription Factors / metabolism
  • Transcription Factors / physiology*
  • Transfection
  • Zinc Fingers*

Substances

  • Cell Cycle Proteins
  • Chemokine CCL5
  • DNA-Binding Proteins
  • KLF13 protein, human
  • Kruppel-Like Transcription Factors
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-rel
  • Repressor Proteins
  • Trans-Activators
  • Transcription Factors

Associated data

  • GENBANK/AF132599