Acute exercise causes mitochondrial DNA deletion in rat skeletal muscle

Muscle Nerve. 1999 Feb;22(2):258-61. doi: 10.1002/(sici)1097-4598(199902)22:2<258::aid-mus15>;2-1.


The present study was conducted to determine the effects of acute overload exercise on mitochondrial DNA and the structure of skeletal muscles. Rats were forced to run for 20 min until reaching complete exhaustion. We detected the large-scale deletion (7052 bp) of mitochondrial DNA by the nested polymerase chain reaction, and also observed mitochondrial ultrastructural changes in the soleus muscle.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • DNA Damage*
  • DNA, Mitochondrial / genetics*
  • Male
  • Microscopy, Electron
  • Mitochondria / ultrastructure
  • Muscle Fatigue / physiology
  • Muscle, Skeletal / pathology
  • Muscle, Skeletal / physiopathology*
  • Muscle, Skeletal / ultrastructure
  • Physical Exertion / physiology*
  • Polymerase Chain Reaction
  • Rats
  • Rats, Wistar


  • DNA, Mitochondrial