Lipopolysaccharide (LPS) from Burkholderia cepacia is more active than LPS from Pseudomonas aeruginosa and Stenotrophomonas maltophilia in stimulating tumor necrosis factor alpha from human monocytes

Infect Immun. 1999 Mar;67(3):1505-7. doi: 10.1128/IAI.67.3.1505-1507.1999.


Whole cells and lipopolysaccharides (LPSs) extracted from Burkholderia cepacia, Pseudomonas aeruginosa, Stenotrophomonas maltophilia, and Escherichia coli were compared in their ability to stimulate tumor necrosis factor alpha (TNF-alpha) from the human monocyte cell line MonoMac-6. B. cepacia LPS, on a weight-for-weight basis, was found to have TNF-alpha-inducing activity similar to that of LPS from E. coli, which was approximately four- and eightfold greater than the activity of LPSs from P. aeruginosa and S. maltophilia, respectively. The LPS-stimulated TNF-alpha production from monocytes was found to be CD14 dependent. These results suggest that B. cepacia LPS might play a role in the pathogenesis of inflammatory lung disease in cystic fibrosis, and in some patients it might be responsible, at least in part, for the sepsis-like cepacia syndrome.

MeSH terms

  • Burkholderia cepacia / pathogenicity*
  • Cell Line
  • Cystic Fibrosis / microbiology
  • Dose-Response Relationship, Drug
  • Humans
  • Lipopolysaccharide Receptors / physiology
  • Lipopolysaccharides / toxicity*
  • Monocytes / metabolism*
  • Pseudomonas aeruginosa / pathogenicity*
  • Tumor Necrosis Factor-alpha / biosynthesis*
  • Xanthomonas / pathogenicity


  • Lipopolysaccharide Receptors
  • Lipopolysaccharides
  • Tumor Necrosis Factor-alpha