Abstract
Expression of Bcl-2, Bax, p53 and induction of apoptosis were studied in cisplatin or Taxol treated monolayer and spheroid cultures of ovarian cancer cell lines (SKOV-3, UL-1, UL-3C). While cisplatin (15-75 microg/ml) induced apoptosis in monolayer and spheroid cultures, Taxol (100-800 nM) induced fragmentation in monolayers only. Cisplatin induced up to 5-fold DNA fragmentation in monolayers, while 3-fold (UL-3C, SKOV-3), and 1.5-fold (UL-1) in spheroids. Taxol treatment of monolayers resulted in the characteristic phosphorylation of Bcl-2, which was not demonstrated in spheroid cultures. Bax expression was reduced in spheroids following cisplatin or Taxol treatment, while p53 levels remained unchanged.
MeSH terms
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Antineoplastic Agents / pharmacology
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Antineoplastic Agents, Phytogenic / pharmacokinetics
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Antineoplastic Agents, Phytogenic / pharmacology*
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Apoptosis
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Cisplatin / pharmacology
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Female
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Humans
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Ovarian Neoplasms / drug therapy*
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Ovarian Neoplasms / metabolism
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Paclitaxel / pharmacokinetics
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Paclitaxel / pharmacology*
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Phosphorylation
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Proto-Oncogene Proteins / biosynthesis
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Proto-Oncogene Proteins c-bcl-2 / biosynthesis
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Proto-Oncogene Proteins c-bcl-2 / metabolism*
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Spheroids, Cellular / drug effects*
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Spheroids, Cellular / metabolism
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Tumor Cells, Cultured
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Tumor Suppressor Protein p53 / biosynthesis
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bcl-2-Associated X Protein
Substances
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Antineoplastic Agents
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Antineoplastic Agents, Phytogenic
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BAX protein, human
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-bcl-2
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Tumor Suppressor Protein p53
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bcl-2-Associated X Protein
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Paclitaxel
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Cisplatin