Establishment and characterization of nurse cell-like stromal cell lines from synovial tissues of patients with rheumatoid arthritis

Arthritis Rheum. 1999 Feb;42(2):221-8. doi: 10.1002/1529-0131(199902)42:2<221::AID-ANR3>3.0.CO;2-N.

Abstract

Objective: To investigate the features of synovial stromal cells established from patients with rheumatoid arthritis (RA), and to define these cells as nurse cells.

Methods: Synovial nurse-like stromal cell lines (RA-SNCs) were established from patients with RA. These cell lines were examined for morphology, pseudoemperipolesis activity, cell surface markers, and cytokine production. The interaction between these RA-SNCs and a synovial tissue B cell clone was also examined.

Results: RA-SNCs had nurse cell activity. They spontaneously produced interleukin-6 (IL-6), IL-8, granulocyte colony-stimulating factor, and granulocyte-macrophage colony-stimulating factor. Furthermore, they produced IL-1beta and tumor necrosis factor alpha and expressed higher levels of the other cytokines after coculture with the B cell clone. Proliferation and Ig production by the B cell clone were dependent on direct contact with RA-SNCs.

Conclusion: These results indicate that the RA-SNCs were nurse cells. The findings suggest that RA-SNCs may play an important role in the pathogenesis of RA by producing large amounts of cytokines and maintaining infiltrating lymphocytes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal / analysis
  • Antigens, Surface / analysis
  • Arthritis, Rheumatoid / immunology*
  • Arthritis, Rheumatoid / pathology
  • B-Lymphocytes / immunology
  • Cell Line
  • Cell Movement / immunology
  • Coculture Techniques
  • Cytokines / analysis
  • Fluorescent Antibody Technique, Indirect
  • Humans
  • Osteoarthritis / immunology
  • Osteoarthritis / pathology
  • Stromal Cells / immunology*
  • Stromal Cells / ultrastructure
  • Synovial Membrane / immunology*
  • Synovial Membrane / pathology

Substances

  • Antibodies, Monoclonal
  • Antigens, Surface
  • Cytokines