High polymorphism level of genomic sequences flanking insertion sites of human endogenous retroviral long terminal repeats

FEBS Lett. 1999 Jan 29;443(3):341-7. doi: 10.1016/s0014-5793(99)00004-6.

Abstract

The polymorphism at the multitude of loci adjacent to human endogenous retrovirus long terminal repeats (LTRs) was analyzed by a technique for whole genome differential display based on the PCR suppression effect that provides selective amplification and display of genomic sequences flanking interspersed repeated elements. This strategy is simple, target-specific, requires a small amount of DNA and provides reproducible and highly informative data. The average frequency of polymorphism observed in the vicinity of the LTR insertion sites was found to be about 12%. The high incidence of polymorphism within the LTR flanks together with the frequent location of LTRs near genes makes the LTR loci a useful source of polymorphic markers for gene mapping.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Chromosome Mapping / methods*
  • DNA Primers
  • Endogenous Retroviruses / genetics*
  • Genetic Markers / genetics
  • Genome, Human
  • Humans
  • Polymerase Chain Reaction / methods*
  • Polymorphism, Genetic*
  • Reproducibility of Results
  • Terminal Repeat Sequences / genetics*
  • Twins, Monozygotic / genetics

Substances

  • DNA Primers
  • Genetic Markers