Abstract
Latrophilin is a brain-specific Ca2+-independent receptor of alpha-latrotoxin, a potent presynaptic neurotoxin. We now report the finding of two novel latrophilin homologues. All three latrophilins are unusual G protein-coupled receptors. They exhibit strong similarities within their lectin, olfactomedin and transmembrane domains but possess variable C-termini. Latrophilins have up to seven sites of alternative splicing; some splice variants contain an altered third cytoplasmic loop or a truncated cytoplasmic tail. Only latrophilin-1 binds alpha-latrotoxin; it is abundant in brain and is present in endocrine cells. Latrophilin-3 is also brain-specific, whereas latrophilin-2 is ubiquitous. Together, latrophilins form a novel family of heterogeneous G protein-coupled receptors with distinct tissue distribution and functions.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alternative Splicing / genetics*
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Amino Acid Sequence
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Animals
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Blotting, Northern
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Brain / metabolism
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Cattle
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Cell Membrane / chemistry
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Cloning, Molecular
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Endocrine Glands / metabolism
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Extracellular Matrix Proteins / chemistry
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Extracellular Matrix Proteins / metabolism
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GTP-Binding Proteins / physiology*
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Gene Expression*
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Gene Library
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Glycoproteins / chemistry
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Glycoproteins / metabolism
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Liver / metabolism
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Molecular Sequence Data
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Organ Specificity
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Protein Isoforms / chemistry
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Protein Isoforms / genetics
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Protein Isoforms / metabolism
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Rats
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Receptors, Peptide / chemistry
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Receptors, Peptide / genetics*
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Receptors, Peptide / isolation & purification
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Receptors, Peptide / metabolism
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Sequence Homology, Amino Acid
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Spider Venoms / metabolism
Substances
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Extracellular Matrix Proteins
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Glycoproteins
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Protein Isoforms
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Receptors, Peptide
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Spider Venoms
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alpha-latrotoxin receptor
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olfactomedin
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alpha-latrotoxin
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GTP-Binding Proteins
Associated data
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GENBANK/AF111069
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GENBANK/AF111070
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GENBANK/AF111071
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GENBANK/AF111072
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GENBANK/AF111073
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GENBANK/AF111074
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GENBANK/AF111075
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GENBANK/AF111076
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GENBANK/AF111077
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GENBANK/AF111078
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GENBANK/AF111079
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GENBANK/AF111080
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GENBANK/AF111081
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GENBANK/AF111082
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GENBANK/AF111083
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GENBANK/AF111084
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GENBANK/AF111085
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GENBANK/AF111086
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GENBANK/AF111087
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GENBANK/AF111088
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GENBANK/AF111089
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GENBANK/AF111090
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GENBANK/AF111091
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GENBANK/AF111092
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GENBANK/AF111093
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GENBANK/AF111094
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GENBANK/AF111095
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GENBANK/AF111096
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GENBANK/AF111097
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GENBANK/AF111098