Zinc-binding site of an S100 protein revealed. Two crystal structures of Ca2+-bound human psoriasin (S100A7) in the Zn2+-loaded and Zn2+-free states

Biochemistry. 1999 Feb 9;38(6):1695-704. doi: 10.1021/bi982483d.


The crystal structure of human psoriasin (S100A7) in the native, calcium-bound form has been determined from two crystal forms of the protein crystallized with and without divalent zinc. The overall structures of the dimeric protein closely resemble the previously determined holmium-substituted structure. The structures also reveal a zinc-binding site of the protein, which is formed by three histidines and an aspartate residue. Together, these residues coordinate the zinc ion in a way similar to the pattern seen in certain metalloproteases and in particular the collagenase family of proteins. Sequence comparison suggests that this zinc site is present in a number of the remaining members of the S100 family. The structure of S100A7 crystallized in the absence of zinc further shows that loss of zinc results in a reorganization of the adjacent empty and distorted EF-hand loop, causing it to resemble a calcium-loaded EF-hand.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Binding Sites
  • Calcium / chemistry
  • Calcium / metabolism*
  • Calcium-Binding Proteins / chemistry*
  • Calcium-Binding Proteins / metabolism
  • Computer Simulation
  • Crystallization
  • Crystallography, X-Ray
  • Humans
  • Metalloendopeptidases / chemistry
  • Models, Molecular
  • Molecular Sequence Data
  • Protein Structure, Secondary
  • S100 Calcium Binding Protein A7
  • S100 Proteins / chemistry
  • S100 Proteins / metabolism
  • Sequence Alignment
  • Zinc / chemistry
  • Zinc / metabolism*


  • Calcium-Binding Proteins
  • S100 Calcium Binding Protein A7
  • S100 Proteins
  • S100A7 protein, human
  • Metalloendopeptidases
  • Zinc
  • Calcium

Associated data

  • PDB/2PSR
  • PDB/3PSR