Rac1 is required for the formation of three germ layers during gastrulation

Oncogene. 1998 Dec 31;17(26):3427-33. doi: 10.1038/sj.onc.1202595.


The Rac1, a member of the Rho family proteins, regulates actin organization of cytoskeleton and cell adhesion. We used genetic analysis to elucidate the role of Rac1 in mouse embryonic development. The rac1 deficient embryos showed numerous cell deaths in the space between the embryonic ectoderm and endoderm at the primitive streak stage. Investigation of the primary epiblast culture isolated from rac1 deficient embryos indicated that Rac1 is involved in lamellipodia formation, cell adhesion and cell migration in vivo. These results suggest that Rac1-mediated cell adhesion is essential for the formation of three germ layers during gastrulation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Adhesion / genetics
  • Cell Movement / genetics
  • Fetal Death / genetics
  • GTP-Binding Proteins / genetics*
  • GTP-Binding Proteins / metabolism
  • Gastrula / cytology*
  • Gastrula / physiology*
  • Gene Expression Regulation, Developmental
  • Genes, Lethal
  • Mice
  • Mice, Knockout
  • rac GTP-Binding Proteins


  • GTP-Binding Proteins
  • rac GTP-Binding Proteins