Preparation of parenchymal and non-parenchymal cells from adult human liver--morphological and biochemical characteristics

J Clin Chem Clin Biochem. 1976 Nov;14(11):527-32. doi: 10.1515/cclm.1976.14.1-12.527.


By perfusion of the isolated human liver with collagenase and hyaluronidase a mixed suspension of various cell types was obtained. Pure parenchymal cells were prepared by differential centrifugation, pure non-parenchymal cells by the use of pronase and subsequent isopycnic centrifugation on metrizamide gradients (50-300 g/l). About 90% of the parenchymal and non-parenchymal cells were viable as judged by trypan blue staining. Non-parenchymal cells were not capable fo gluconeogenesis but at high rates. Parenchymal cells retained their ability to form glucose and to accumulate glycogen from fructose greater than lactate/pyruvate greater than alanine. Studies on binding of 125I-labelled insulin by isolated parenchymal cells were performed at 30 degrees C. The binding data may fit with a minimum of two classes of binding sites: (a) high affinity--low capacity sties (Kd approximately 6.6 nmol/l, capacity approximately 16 000 insulin molecules per cell) and (b) low affinity-high capacity sites (Kd approsimately 0.37 mumol/l, capacity approximately 646 000 molecules per cell).

MeSH terms

  • Adult
  • Cell Separation / methods
  • Gluconeogenesis
  • Humans
  • Hydrolases
  • Liver / cytology*
  • Liver / metabolism
  • Liver Glycogen / biosynthesis
  • Receptor, Insulin / analysis
  • Ultracentrifugation / methods


  • Liver Glycogen
  • Receptor, Insulin
  • Hydrolases