Presenilins interact with armadillo proteins including neural-specific plakophilin-related protein and beta-catenin

J Neurochem. 1999 Mar;72(3):999-1008. doi: 10.1046/j.1471-4159.1999.0720999.x.

Abstract

Missense substitutions in the presenilin 1 (PS1) and presenilin 2 (PS2) proteins are associated with early-onset familial Alzheimer's disease. We have used yeast-two-hybrid and coimmunoprecipitation methods to show that the large cytoplasmic loop domains of PS1 and PS2 interact specifically with three members of the armadillo protein family, including beta-catenin, p0071, and a novel neuronal-specific armadillo protein--neural plakophilin-related armadillo protein (NPRAP). The PS1:NPRAP interaction occurs between the arm repeats of NPRAP and residues 372-399 at the C-terminal end of the large cytoplasmic loop of PS1. The latter residues contain a single arm-like domain and are highly conserved in the presenilins, suggesting that they form a functional armadillo protein binding site for the presenilins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alzheimer Disease / genetics
  • Amino Acid Sequence
  • Animals
  • Armadillo Domain Proteins
  • Catenins
  • Cell Adhesion Molecules
  • Cells, Cultured
  • Chromatography, Affinity
  • Cytoskeletal Proteins / metabolism*
  • Humans
  • Immunohistochemistry
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism*
  • Mice
  • Microscopy, Confocal
  • Molecular Sequence Data
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / metabolism*
  • Phosphoproteins
  • Plakophilins
  • Precipitin Tests
  • Presenilin-1
  • Presenilin-2
  • Protein Binding
  • Trans-Activators*
  • Transfection
  • beta Catenin

Substances

  • Armadillo Domain Proteins
  • CTNNB1 protein, human
  • CTNNB1 protein, mouse
  • Catenins
  • Cell Adhesion Molecules
  • Cytoskeletal Proteins
  • Membrane Proteins
  • Nerve Tissue Proteins
  • PKP4 protein, human
  • PSEN1 protein, human
  • PSEN2 protein, human
  • Phosphoproteins
  • Plakophilins
  • Presenilin-1
  • Presenilin-2
  • Trans-Activators
  • beta Catenin
  • delta catenin