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. 1999 Mar 15;27(6):1558-60.
doi: 10.1093/nar/27.6.1558.

Amplification of cDNA Ends Based on Template-Switching Effect and Step-Out PCR

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Free PMC article

Amplification of cDNA Ends Based on Template-Switching Effect and Step-Out PCR

M Matz et al. Nucleic Acids Res. .
Free PMC article

Abstract

A new method for amplifying cDNA ends is described which requires only first-strand cDNA synthesis and a single PCR to generate a correct product with very low or no background. The method can be successfully applied to total RNA as well as poly A+ RNA. The same first-strand cDNA can be used to amplify flanking sequences of any cDNA species present in the sample.

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