Cell surface distribution of lectin receptors determined by resonance energy transfer

Nature. 1976 Dec 2;264(5585):411-5. doi: 10.1038/264411a0.


The surface topography of concanavalin A (con A) bound to normal and transformed murine fibroblasts has been studied by a new technique involving fluorescence resonance energy transfer (RET), RET can provide a high resolution "map" of the distances separating con A-receptor complexes in single living cells. The distribution of con A is non-random in both normal and transformed cells, but sites are more closely approximated in the transformed. Approximation is induced by the con A but occurs at extremely slow rates indicating that the topography is not primarily determined by simple diffusion of complexes.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cell Line
  • Cell Membrane / metabolism*
  • Cell Membrane / ultrastructure
  • Cell Transformation, Neoplastic*
  • Diffusion
  • Energy Transfer
  • Fibroblasts
  • Microscopy, Fluorescence
  • Movement
  • Receptors, Concanavalin A / metabolism*
  • Receptors, Drug / metabolism*


  • Receptors, Concanavalin A
  • Receptors, Drug