2D protein electrophoresis: can it be perfected?

Curr Opin Biotechnol. 1999 Feb;10(1):16-21. doi: 10.1016/s0958-1669(99)80004-4.


23 years after O'Farrel developed two-dimensional gel electrophoresis we still debate if the technique can be improved, or if there are other alternative separation technologies that can challenge its central position in proteomic projects. These questions are relevant as the pharmaceutical industry expects proteomic studies to provide novel protein targets for drug discovery and diagnostics. In our opinion, there are various aspects of the technology that can be improved, including resolution, sample preparation and detection, but so far there is no alternative technique(s) available, or any under development, that can replace it.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Biochemistry / methods
  • Electrophoresis, Gel, Two-Dimensional / methods*
  • Eukaryotic Cells / chemistry
  • Humans
  • Proteins / analysis
  • Proteins / chemistry


  • Proteins