Structure and mechanism in site-specific recombination

Curr Opin Struct Biol. 1999 Feb;9(1):14-20. doi: 10.1016/s0959-440x(99)80003-7.

Abstract

Three-dimensional structural information on the integrase family of site-specific recombinases has only recently become available, with the crystal structures of catalytic domains, full-length proteins and protein-DNA complexes of this family reported over the past two years. These results have led to a model for the overall architecture and active site stereochemistry of the recombination pathway that addresses a number of interesting mechanistic issues.

Publication types

  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Binding Sites / genetics
  • DNA / chemistry
  • DNA / genetics
  • DNA / metabolism
  • DNA Nucleotidyltransferases / chemistry
  • DNA Nucleotidyltransferases / genetics
  • DNA Nucleotidyltransferases / metabolism
  • Integrases / chemistry
  • Integrases / genetics*
  • Integrases / metabolism*
  • Models, Genetic
  • Models, Molecular
  • Nucleic Acid Conformation
  • Protein Conformation
  • Recombinases
  • Recombination, Genetic*
  • Stereoisomerism
  • Tyrosine / chemistry
  • Viral Proteins*

Substances

  • Recombinases
  • Viral Proteins
  • Tyrosine
  • DNA
  • Cre recombinase
  • DNA Nucleotidyltransferases
  • Integrases
  • integron integrase IntI1