Posterior vitreous detachment induced by injection of plasmin and sulfur hexafluoride in the rabbit vitreous

Retina. 1999;19(1):55-8. doi: 10.1097/00006982-199901000-00009.


Purpose: To investigate whether an injection of plasmin and sulfur hexafluoride (SF6) can induce posterior vitreous detachment (PVD) without vitrectomy.

Methods: One eye each of 15 New Zealand white rabbits was assigned to one of three groups. Eyes in group 1 received a vitreous injection of 1 unit of human plasmin (0.1 mL reconstituted in balanced salt solution) and 0.5 mL of SF6; eyes in group 2 received a vitreous injection of plasmin alone; eyes in group 3 received a vitreous injection of SF6 alone. Seven days after injection, all animals were monitored electroretinographically and killed, and the eyes were enucleated. After fixation, scanning electron microscopy was performed.

Results: In group 1 eyes, the retinal surface was smooth except for the vitreous base, which showed complete separation of the vitreous cortex from the retina, indicating PVD. In group 2 and 3 eyes, sparse collagen fibers remained on the retinal surface.

Conclusion: Vitreous injection of plasmin combined with SF6 can induce PVD without vitrectomy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Drug Therapy, Combination
  • Electroretinography
  • Eye Diseases / pathology
  • Eye Diseases / surgery*
  • Fibrinolysin / administration & dosage*
  • Injections
  • Male
  • Microscopy, Electron, Scanning
  • Ophthalmologic Surgical Procedures / methods*
  • Rabbits
  • Retina / physiology
  • Sulfur Hexafluoride / administration & dosage*
  • Vitreous Body / drug effects
  • Vitreous Body / surgery*
  • Vitreous Body / ultrastructure


  • Fibrinolysin
  • Sulfur Hexafluoride