Cloning and characterization of two cytoplasmic dynein intermediate chain genes in mouse and human

Genomics. 1999 Feb 1;55(3):257-67. doi: 10.1006/geno.1998.5665.

Abstract

Cytoplasmic dynein is a large multisubunit microtubule-based motor protein, which mediates movement of numerous intracellular organelles. We report here the identification of the human homologue of cytoplasmic dynein intermediate chain 1 gene (DNCI1) located on human chromosome 7q21.3-q22.1. The mouse orthologue (Dnci1) was identified along with another highly related gene, Dnci2, and their RNA in situ expression patterns were examined during mouse embryogenesis. Dnci1 was found to have a highly restricted expression domain in the developing forebrain as well as the peripheral nervous system (PNS), while Dnci2 displayed a broad expression profile throughout the entire central nervous system and most of the PNS. A dynamic expression profile was also found for Dnci2 in the developing mouse limb bud. The data presented here provide a framework for the further analysis of the functional role of Dnci1 and Dnci2 in mouse and DNCI1 in human.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Chromosomes, Artificial, Yeast
  • Dyneins / genetics*
  • Dyneins / metabolism*
  • Dyneins / physiology
  • Embryo, Mammalian / metabolism
  • Embryonic and Fetal Development
  • Humans
  • In Situ Hybridization
  • Limb Buds / metabolism
  • Mice
  • Models, Genetic
  • Molecular Sequence Data
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Homology, Amino Acid
  • Time Factors
  • Tissue Distribution

Substances

  • Dyneins

Associated data

  • GENBANK/AF063228
  • GENBANK/AF063229
  • GENBANK/AF063230
  • GENBANK/AF063231