A Restriction Endonuclease From Staphylococcus Aureus

Nucleic Acids Res. 1976 Nov;3(11):3193-202. doi: 10.1093/nar/3.11.3193.


A specific endonuclease, Sau 3AI, has been partially purified from Staphylococcus aureus strain 3A by DEAE-cellulose chromatography. The enzyme cleaves adenovirus type 5 DNA many times, SV40 DNA eight times but does not cleave double-stranded phi X174 DNA. It recognizes the sequence (see article) and cleaves as indicated by the arrows. Evidence is presented that this enzyme plays a role in the biological restriction-modification system of Staphylococcus aureus strain 3A.

MeSH terms

  • Base Sequence
  • DNA
  • DNA Restriction Enzymes* / isolation & purification
  • DNA Restriction Enzymes* / metabolism
  • Endonucleases* / metabolism
  • Molecular Weight
  • Staphylococcus aureus / enzymology*


  • DNA
  • Endonucleases
  • DNA Restriction Enzymes