gigas, a Drosophila homolog of tuberous sclerosis gene product-2, regulates the cell cycle

Cell. 1999 Feb 19;96(4):529-39. doi: 10.1016/s0092-8674(00)80657-1.

Abstract

Tuberous sclerosis complex (TSC) is an autosomal dominant disorder leading to the widespread development of benign tumors that often contain giant cells. We show that the Drosophila gene gigas encodes a homolog of TSC2, a gene mutated in half of TSC patients. Clones of gigas mutant cells induced in imaginal discs differentiate normally to produce adult structures. However, the cells in these clones are enlarged and repeat S phase without entering M phase. Our results suggest that the TSC disorder may result from an underlying defect in cell cycle control. We have also identified a Drosophila homolog of TSC1.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alleles
  • Animals
  • Cell Cycle / genetics
  • Cell Cycle Proteins / genetics*
  • Cell Size
  • Cloning, Molecular
  • Cyclin A / metabolism
  • Cyclin B / metabolism
  • Cyclins / metabolism
  • Drosophila / genetics*
  • Drosophila / growth & development
  • Drosophila / metabolism
  • Drosophila Proteins*
  • Eye / cytology
  • Eye / growth & development
  • Eye / metabolism
  • Female
  • Homozygote
  • Male
  • Mitosis / genetics
  • Molecular Sequence Data
  • Phenotype
  • Repressor Proteins / genetics*
  • S Phase / genetics
  • Sequence Homology, Amino Acid
  • Tuberous Sclerosis / genetics*
  • Tuberous Sclerosis Complex 2 Protein
  • Tumor Suppressor Proteins

Substances

  • Cell Cycle Proteins
  • CycB protein, Drosophila
  • Cyclin A
  • Cyclin B
  • Cyclins
  • Drosophila Proteins
  • Repressor Proteins
  • Tuberous Sclerosis Complex 2 Protein
  • Tumor Suppressor Proteins
  • gig protein, Drosophila

Associated data

  • GENBANK/AF172995
  • GENBANK/AF173560