High-performance liquid chromatographic determination of paclitaxel in rat serum: application to a toxicokinetic study

J Chromatogr B Biomed Sci Appl. 1999 Jan 22;721(2):317-20. doi: 10.1016/s0378-4347(98)00465-4.

Abstract

A simple and sensitive high-performance liquid chromatographic method is described for the determination of paclitaxel (Taxol) at 230 nm using a Nucleosil C18 (5 microm) column and a methanol-water (70:30, v/v) mobile phase following a single-step extraction from serum with dichloromethane. The assay was validated against the classical criteria and was applied to a toxicokinetic study in rats after one or five, one per week) intraperitoneal administrations of 16 mg/kg Taxol.

MeSH terms

  • Animals
  • Antineoplastic Agents, Phytogenic / blood*
  • Antineoplastic Agents, Phytogenic / pharmacokinetics*
  • Antineoplastic Agents, Phytogenic / toxicity
  • Chromatography, High Pressure Liquid
  • Injections, Intraperitoneal
  • Paclitaxel / blood*
  • Paclitaxel / pharmacokinetics*
  • Paclitaxel / toxicity
  • Rats
  • Rats, Sprague-Dawley
  • Reproducibility of Results

Substances

  • Antineoplastic Agents, Phytogenic
  • Paclitaxel