Autoantibodies to molecular targets in neutrophils in patients with ulcerative colitis

Dig Dis Sci. 1999 Feb;44(2):415-23. doi: 10.1023/a:1026683224057.


Autoantibodies against neutrophil granulocytes are frequently observed in patients with ulcerative colitis, but a precise description of the autoantigens involved is lacking. Therefore, sera from 75 patients with ulcerative colitis were studied for antibody specificities by means of indirect immunofluorescence microscopy, enzyme-linked immunosorbent assays, and immunoblotting of extracts of neutrophils, neutrophil granules and lymphocytes. Fifty-six percent of the sera reacted in indirect immunofluorescence with ethanol-fixed neutrophils. On formalin-fixed cells most sera shifted fluorescence pattern, indicating a cytoplasmic origin of antigen(s). Only a few sera reacted with specific, known antigens. Immunoblots showed reactions with a broad panel of antigens with preferences for proteins around 55-65 kDa, which were present in azurophilic granules and in cytosol, an 80-kDa protein found in the specific granules, and a 110-kDa unknown cytosol component. In conclusion, neutrophil-specific IgG autoantibodies from ulcerative colitis patients react with several different antigens, most of which are of nonnuclear origin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Autoantibodies / blood*
  • Autoantigens / analysis
  • Colitis, Ulcerative / immunology*
  • Cytoplasm / immunology
  • Cytosol / immunology
  • Enzyme-Linked Immunosorbent Assay
  • Epitopes
  • Fluorescent Antibody Technique, Indirect
  • Humans
  • Immunoblotting
  • Immunoglobulin G / analysis
  • Lymphocytes / immunology
  • Neutrophils / immunology*


  • Autoantibodies
  • Autoantigens
  • Epitopes
  • Immunoglobulin G