Variation of liver-type fatty acid binding protein content in the human hepatoma cell line HepG2 by peroxisome proliferators and antisense RNA affects the rate of fatty acid uptake

Biochim Biophys Acta. 1999 Feb 25;1437(2):194-201. doi: 10.1016/s1388-1981(99)00008-6.


The liver-type fatty acid binding protein (L-FABP), a member of a family of mostly cytosolic 14-15 kDa proteins known to bind fatty acids in vitro and in vivo, is discussed to play a role in fatty acid uptake. Cells of the hepatoma HepG2 cell line endogenously express this protein to approximately 0.2% of cytosolic proteins and served as a model to study the effect of L-FABP on fatty acid uptake, by manipulating L-FABP expression in two approaches. First, L-FABP content was more than doubled upon treating the cells with the potent peroxisome proliferators bezafibrate and Wy14,643 and incubation of these cells with [1-14C]oleic acid led to an increase in fatty acid uptake rate from 0.55 to 0.74 and 0.98 nmol/min per mg protein, respectively. In the second approach L-FABP expression was reduced by stable transfection with antisense L-FABP mRNA yielding seven clones with L-FABP contents ranging from 0.03% to 0.14% of cytosolic proteins. This reduction to one sixth of normal L-FABP content reduced the rate of [1-14C]oleic acid uptake from 0.55 to 0. 19 nmol/min per mg protein, i.e., by 66%. The analysis of peroxisome proliferator-treated cells and L-FABP mRNA antisense clones revealed a direct correlation between L-FABP content and fatty acid uptake.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bezafibrate / pharmacology
  • Carcinoma, Hepatocellular
  • Carrier Proteins / biosynthesis*
  • Carrier Proteins / genetics
  • Fatty Acid-Binding Protein 7
  • Fatty Acid-Binding Proteins
  • Fatty Acids / metabolism*
  • Humans
  • Myelin P2 Protein / biosynthesis*
  • Myelin P2 Protein / genetics
  • Neoplasm Proteins*
  • Peroxisome Proliferators / pharmacology*
  • Pyrimidines / pharmacology
  • RNA, Antisense
  • Transfection
  • Tumor Cells, Cultured
  • Tumor Suppressor Proteins*


  • Carrier Proteins
  • FABP1 protein, human
  • FABP7 protein, human
  • Fabp1 protein, mouse
  • Fatty Acid-Binding Protein 7
  • Fatty Acid-Binding Proteins
  • Fatty Acids
  • Myelin P2 Protein
  • Neoplasm Proteins
  • Peroxisome Proliferators
  • Pyrimidines
  • RNA, Antisense
  • Tumor Suppressor Proteins
  • pirinixic acid
  • Bezafibrate