Reversible inactivation of subcortical sites by drug injection

J Neurosci Methods. 1999 Jan;86(2):119-28. doi: 10.1016/s0165-0270(98)00161-7.


Reversible inactivation of subcortical targets by means of drug injections has been a powerful tool for revealing the contributions of discrete brain structures to behavior and the functional organization of the brain. This paper is intended to provide practical advice on this approach, including the choice of drug, means of delivering drugs, strategies for evaluating the action of injected drugs, and the application of this method to experiments with awake animals.

Publication types

  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Anesthetics, Local / administration & dosage
  • Anesthetics, Local / pharmacology
  • Animals
  • Brain / drug effects*
  • Brain / physiology*
  • Microinjections / instrumentation
  • Microinjections / methods*
  • Pharmaceutical Preparations / administration & dosage*


  • Anesthetics, Local
  • Pharmaceutical Preparations