The nitrate assimilation pathway has been the matter of intensive research during the past decade. Many genes involved in low and high affinity nitrate uptake have been identified in fungi, algae and, more recently, in plants. The plant genes so far isolated are transcriptionally regulated; their inducibility by nitrate seems to be a common feature, shared by their homologs in fungi and algae. A number of questions remain to be elucidated regarding the physiological roles of these transporters and the regulation of their expression.