DNA is as effective as protein at inducing antibody in fish

Vaccine. 1999 Feb 26;17(7-8):965-72. doi: 10.1016/s0264-410x(98)00312-0.


Antiviral vaccines are needed for fish. 50 microg plasmid DNA in saline by the intramuscular route and 10 microg beta-gal protein in a commercial oil adjuvant by the peritoneal route induced serum antibody of the same titre and avidity in goldfish. The DNA expressed beta-gal under control of the immediate early promoter/enhancer gene of human cytomegalovirus. Commercial bacterin vaccines are administered to fish by the intraperitoneal route with oil and this route for DNA induced only 2-fold less antibody than DNA by the intramuscular route. Bacterin vaccines and antiviral plasmid DNA could therefore be co-injected into the peritoneum of fish in an oil adjuvant as a single dose.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Viral / biosynthesis*
  • Antibodies, Viral / blood
  • Antibody Affinity
  • Dose-Response Relationship, Immunologic
  • Goldfish / immunology*
  • Humans
  • Injections, Intramuscular / veterinary
  • Injections, Intraperitoneal / veterinary
  • Muscle, Skeletal / immunology
  • Plasmids / administration & dosage
  • Plasmids / immunology
  • Vaccines, DNA / administration & dosage
  • Vaccines, DNA / genetics
  • Vaccines, DNA / immunology*
  • beta-Galactosidase / administration & dosage
  • beta-Galactosidase / genetics
  • beta-Galactosidase / immunology*


  • Antibodies, Viral
  • Vaccines, DNA
  • beta-Galactosidase