Protective immune response against Streptococcus pyogenes in mice after intranasal vaccination with the fibronectin-binding protein SfbI

J Infect Dis. 1999 Apr;179(4):901-6. doi: 10.1086/314655.

Abstract

Despite the significant impact on human health of Streptococcus pyogenes, an efficacious vaccine has not yet been developed. Here, the potential as a vaccine candidate of a major streptococcal adhesin, the fibronectin-binding protein SfbI, was evaluated. Intranasal immunization of mice with either SfbI alone or coupled to cholera toxin B subunit (CTB) triggered efficient SfbI-specific humoral (mainly IgG) and lung mucosal (14% of total IgA) responses. CTB-immunized control mice were not protected against challenge with S. pyogenes (90%-100% lethality), whereas SfbI-vaccinated animals showed 80% and 90% protection against homologous and heterologous challenge, respectively. Multiple areas of consolidation with diffused cellular infiltrates (macrophages and neutrophils) were observed in lungs from control mice; the histologic structure was preserved in SfbI-vaccinated animals, which occasionally presented focal infiltrates confined to the perivascular, peribronchial, and subpleural areas. These results suggest that SfbI is a promising candidate for inclusion in acellular vaccines against S. pyogenes.

MeSH terms

  • Adhesins, Bacterial*
  • Administration, Intranasal
  • Animals
  • Antibodies, Bacterial / blood
  • Bacterial Proteins / immunology*
  • Bacterial Vaccines / immunology*
  • Carrier Proteins / immunology*
  • Female
  • Immunity, Mucosal
  • Lung / pathology
  • Mice
  • Mice, Inbred BALB C
  • Streptococcus pyogenes / immunology*
  • Vaccination

Substances

  • Adhesins, Bacterial
  • Antibodies, Bacterial
  • Bacterial Proteins
  • Bacterial Vaccines
  • Carrier Proteins
  • fibronectin-binding proteins, bacterial