Cyclin A1 expression in leukemia and normal hematopoietic cells

Blood. 1999 Mar 15;93(6):2067-74.

Abstract

Human cyclin A1 is a newly cloned, tissue-specific cyclin that is prominently expressed in normal testis. In this study, we showed that cyclin A1 was highly expressed in a subset of leukemia samples from patients. The highest frequency of cyclin A1 overexpression was observed in acute myelocytic leukemias, especially those that were at the promyelocyte (M3) and myeloblast (M2) stages of development. Cyclin A1 expression was also detected in normal CD34(+) progenitor cells. The expression of cyclin A1 increased when these cells were stimulated to undergo myeloid differentiation in vitro. Taken together, our observations suggest that cyclin A1 may have a role in hematopoiesis. High levels of cyclin A1 expression are especially associated with certain leukemias blocked at the myeloblast and promyelocyte stages of differentiation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antigens, CD34 / analysis
  • Blotting, Northern
  • Cell Differentiation
  • Cyclin A / analysis
  • Cyclin A / genetics*
  • Cyclin A1
  • Flow Cytometry
  • Fluorescent Antibody Technique
  • Gene Expression*
  • Hematopoietic Stem Cells / immunology
  • Hematopoietic Stem Cells / metabolism*
  • Humans
  • Leukemia / metabolism*
  • Leukemia, Myeloid, Acute / metabolism
  • Leukemia, Promyelocytic, Acute / metabolism
  • RNA, Messenger / analysis
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • Antigens, CD34
  • CCNA1 protein, human
  • Cyclin A
  • Cyclin A1
  • RNA, Messenger