The putative regulator of catechol catabolism in Rhodococcus opacus 1CP--an IclR-type, not a LysR-type transcriptional regulator

Antonie Van Leeuwenhoek. Jul-Oct 1998;74(1-3):71-82. doi: 10.1023/a:1001755928637.

Abstract

The catechol catabolic genes catABC from Rhodococcus opacus 1CP have previously been characterized by sequence analysis of the insert cloned on plasmid pRER1. Now, a 5.1-kb DNA fragment which overlaps with the insert of pRER1 was cloned, yielding pRER2, and subjected to sequencing. Besides three other open reading frames, a gene was detected ca 200 bp upstream of the catechol 1,2-dioxygenase gene catA, which is obviously transcribed divergently from catABC. The protein which can be deduced from this gene, CatR, resembles members of the PobR subfamily of IclR-type regulatory proteins. This finding was unexpected, as all catechol and chlorocatechol gene clusters known thus far from proteobacteria are under control of LysR-type regulators. It was not possible to inactivate catR by homologous recombination. However, heterologously expressed CatR in vitro bound specifically to the intergenic region between catR and catA thereby providing a first indication for a possible involvement of CatR in the regulation of catechol catabolism.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / genetics*
  • Base Sequence
  • Catechol 1,2-Dioxygenase
  • Catechols / metabolism*
  • Cloning, Molecular
  • DNA, Bacterial / metabolism
  • DNA-Binding Proteins / classification
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism
  • Dioxygenases*
  • Enzyme Induction
  • Escherichia coli Proteins*
  • Molecular Sequence Data
  • Oxygenases / biosynthesis
  • Protein Binding
  • Repressor Proteins / genetics*
  • Rhodococcus / genetics*
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Transcription Factors / classification
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism

Substances

  • Bacterial Proteins
  • Catechols
  • DNA, Bacterial
  • DNA-Binding Proteins
  • Escherichia coli Proteins
  • Repressor Proteins
  • Transcription Factors
  • CatR protein, bacteria
  • IclR protein, E coli
  • LysR protein, Bacteria
  • Oxygenases
  • Dioxygenases
  • Catechol 1,2-Dioxygenase

Associated data

  • GENBANK/X99622