A genome-wide screen for asthma-associated quantitative trait loci in a mouse model of allergic asthma

Hum Mol Genet. 1999 Apr;8(4):601-5. doi: 10.1093/hmg/8.4.601.

Abstract

Asthma is the most common illness of childhood, affecting one child in seven in the UK. Asthma has a genetic basis, but genetic studies of asthma in humans are confounded by uncontrolled environmental factors, varying penetrance and phenotypic pleiotropy. An animal model of asthma would offer controlled exposure, limited and consistent genetic variation, and unlimited size of sibships. Following immunization and subsequent challenge with ovalbumin, the Biozzi BP2 mouse shows features of asthma, including airway inflammation, eosinophil infiltration and non-specific bronchial responsiveness. In order to identify genetic loci influencing these traits, a cross was made between BP2 and BALB/c mice, and a genome-wide screen carried out in the F2progeny of the F1intercross. Five potentially linked loci were identified, four of which corresponded to human regions of syntenic homology that previously have shown linkage to asthma-associated traits.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Airway Resistance / drug effects
  • Allergens / adverse effects
  • Allergens / immunology
  • Animals
  • Asthma / etiology
  • Asthma / genetics*
  • Asthma / pathology
  • Bronchi / cytology
  • Bronchi / drug effects
  • Bronchi / pathology
  • Bronchoalveolar Lavage Fluid / cytology
  • Chromosomes / genetics
  • Crosses, Genetic
  • Disease Models, Animal
  • Eosinophils / cytology
  • Epithelial Cells / cytology
  • Epithelial Cells / drug effects
  • Female
  • Genetic Linkage
  • Genetic Testing
  • Genome*
  • Leukocyte Count / drug effects
  • Lod Score
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Nasal Provocation Tests
  • Ovalbumin / adverse effects
  • Ovalbumin / immunology
  • Quantitative Trait, Heritable*

Substances

  • Allergens
  • Ovalbumin