Cutting edge: secondary lymphoid-tissue chemokine (SLC) and CC chemokine receptor 7 (CCR7) participate in the emigration pathway of mature dendritic cells from the skin to regional lymph nodes

J Immunol. 1999 Mar 1;162(5):2472-5.


Dendritic cells (DCs) emigrate to regional lymph nodes (LNs) during immune responses via afferent lymphatic channels. Secondary lymphoid-tissue chemokine (SLC), a CC chemokine, is expressed in secondary lymphoid organs and mediates the chemotaxis of lymphocytes and DCs via its receptor, CC chemokine receptor 7 (CCR7). By dual-label fluorescence confocal microscopy, we showed MHC class II-positive cells within SLC-staining lymphatic channels in the mouse dermis. SLC was a potent in vitro chemoattractant for cultured, migratory skin DCs, and it enhanced the emigration of MHC class II-positive DCs from mouse skin explants by an average of 2.5-fold. Mature or cytokine-activated, but not resting, Langerhans cells expressed CCR7 mRNA by RT-PCR. Anti-SLC Abs, but not control or anti-eotaxin Abs, blocked the in vivo migration of 51Cr-labeled, skin-derived DCs from footpads to draining LNs by 50% (n = 9, p < 0. 005). Thus, we provide direct evidence that SLC and CCR7 participate in the emigration of DCs from peripheral tissue to LNs via lymphatics.

MeSH terms

  • Animals
  • Cell Movement
  • Chemokine CCL21
  • Chemokines, CC / physiology*
  • Dendritic Cells / physiology*
  • Female
  • Lymph Nodes / immunology*
  • Mice
  • Mice, Inbred BALB C
  • Receptors, CCR7
  • Receptors, Chemokine / physiology*
  • Skin / immunology*
  • Tumor Necrosis Factor-alpha / pharmacology


  • Ccl21c protein, mouse
  • Ccr7 protein, mouse
  • Chemokine CCL21
  • Chemokines, CC
  • Receptors, CCR7
  • Receptors, Chemokine
  • Tumor Necrosis Factor-alpha