Characterization of a murine gene homologous to the bovine CaCC chloride channel

Gene. 1999 Mar 4;228(1-2):181-8. doi: 10.1016/s0378-1119(98)00620-9.

Abstract

The bovine CaCC protein is a putative Ca2+-dependent Cl- channel of airway epithelial cells. Therefore, CaCC proteins could contribute to transepithelial Cl- transport and accordingly modify the phenotype of cystic fibrosis (CF) patients. We have identified a murine EST containing a full-length cDNA coding for a 902-amino-acid protein highly homologous to bovine CaCC. The murine gene (mCaCC) maps to chromosome 3 at the H2-H3 band and is expressed, as indicated by Northern blot analysis, in mouse skin and kidney but not in brain, heart, lung or testis. RT-PCR indicates a low expression in tracheal epithelial cells. Heterologous expression of mCaCC in Xenopus oocytes elicits membrane currents that are anion-selective and inhibited by DIDS and by niflumic acid, a blocker of the endogenous chloride current in oocytes. The identification of genes belonging to the CaCC family will help to evaluate their role as ion channels or channel regulators and their actual contribution to epithelial chloride transport.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / pharmacology
  • Amino Acid Sequence
  • Animals
  • Blotting, Northern
  • Calcium / physiology*
  • Cattle
  • Chloride Channels / administration & dosage
  • Chloride Channels / genetics*
  • Chromosome Mapping
  • Expressed Sequence Tags
  • Female
  • Gene Expression
  • Genes / genetics*
  • In Situ Hybridization, Fluorescence
  • Ionomycin / pharmacology
  • Ionophores / pharmacology
  • Male
  • Membrane Potentials / drug effects
  • Mice
  • Microinjections
  • Molecular Sequence Data
  • Niflumic Acid / pharmacology
  • Oocytes / cytology
  • Oocytes / drug effects
  • Oocytes / physiology
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Sodium Chloride / pharmacology
  • Tissue Distribution
  • Xenopus

Substances

  • Chloride Channels
  • Ionophores
  • RNA, Messenger
  • Sodium Chloride
  • Niflumic Acid
  • Ionomycin
  • 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
  • Calcium

Associated data

  • GENBANK/AF052746