A missense mutation accounts for the defect in the glycerol-3-phosphate acyltransferase expressed in the plsB26 mutant

J Bacteriol. 1999 Mar;181(6):1944-6. doi: 10.1128/JB.181.6.1944-1946.1999.

Abstract

The sn-glycerol-3-phosphate acyltransferase (plsB) catalyzes the first step in membrane phospholipid formation. A conditional Escherichia coli mutant (plsB26) has a single missense mutation (G1045A) predicting the expression of an acyltransferase with an Ala349Thr substitution. The PlsB26 protein had a significantly reduced glycerol-3-phosphate acyltransferase specific activity coupled with an elevated Km for glycerol-3-phosphate.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alleles
  • Base Sequence
  • DNA Primers / genetics
  • DNA, Bacterial / genetics
  • Escherichia coli / enzymology*
  • Escherichia coli / genetics*
  • Gene Expression
  • Genes, Bacterial
  • Glycerol-3-Phosphate O-Acyltransferase / genetics*
  • Glycerol-3-Phosphate O-Acyltransferase / metabolism*
  • Kinetics
  • Molecular Sequence Data
  • Mutation, Missense*

Substances

  • DNA Primers
  • DNA, Bacterial
  • Glycerol-3-Phosphate O-Acyltransferase

Associated data

  • GENBANK/AF106625