The present study was done both in vivo by cannulating pancreatic duct of morphine-dependent rats and in vitro using perfused pancreatic slices from morphine-dependent rats. The results were as follows: (1) In morphine-dependent rat the pancreatic amylase secretion induced by CCK-8 was significantly declined; (2) Amylase content of pancreatic tissue in morphine-dependent rats was lower than that of normal rats. It is suggested that the synthesis of amylase in morphine-dependent rat pancreas may be inhibited.