FGF8 functions in the specification of the right body side of the chick

Curr Biol. 1999 Mar 11;9(5):277-80. doi: 10.1016/s0960-9822(99)80119-5.

Abstract

Left-right asymmetry in vertebrate embryos is first recognisable using molecular markers that encode secreted proteins or transcription factors. The asymmetry becomes morphologically obvious in the turning of the embryo and in the development of the heart, the gut and other visceral organs. In the chick embryo, a signalling pathway for the specification of the left body side was demonstrated. Here, Sonic hedgehog (Shh) protein is the first asymmetric signal identified in the node [1] [2]. Further downstream in this pathway are the left-specific genes nodal, lefty-1, lefty-2 and Pitx2 [1] [3] [4] [5]. On the right body side, a function of the activin pathway is indicated by the right-sided expression of cActRIIa [1] [6]. We detected that another key molecule in vertebrate development, fibroblast growth factor 8 (FGF8) [7] [8], is expressed asymmetrically on the right side of the posterior node. We demonstrate that transcription of FGF8 is induced by activin and the FGF8 protein inhibits the expression of nodal and Pitx2 and leads to expression of the chicken snail related gene (cSnR) [9]. Left-sided application of FGF8 randomises the direction of heart looping.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Body Patterning / physiology*
  • Chick Embryo
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism
  • Fibroblast Growth Factor 8
  • Fibroblast Growth Factors / genetics
  • Fibroblast Growth Factors / metabolism
  • Fibroblast Growth Factors / physiology*
  • Hedgehog Proteins
  • Homeodomain Proteins / genetics
  • Homeodomain Proteins / metabolism
  • Nodal Protein
  • Nuclear Proteins*
  • Paired Box Transcription Factors
  • Proteins / genetics
  • Proteins / metabolism
  • Signal Transduction / physiology*
  • Snail Family Transcription Factors
  • Trans-Activators*
  • Transcription Factors / genetics
  • Transcription Factors / metabolism
  • Transforming Growth Factor beta / genetics
  • Transforming Growth Factor beta / metabolism

Substances

  • DNA-Binding Proteins
  • Hedgehog Proteins
  • Homeodomain Proteins
  • Nodal Protein
  • Nuclear Proteins
  • Paired Box Transcription Factors
  • Proteins
  • Snail Family Transcription Factors
  • Trans-Activators
  • Transcription Factors
  • Transforming Growth Factor beta
  • homeobox protein PITX1
  • homeobox protein PITX3
  • snail-related protein, Gallus gallus
  • Fibroblast Growth Factor 8
  • homeobox protein PITX2
  • Fibroblast Growth Factors