Spectrum of transforming sequences detected by tumorigenicity assay in a large series of human neoplasms

Int J Cancer. 1999 Mar 15;80(6):857-62. doi: 10.1002/(sici)1097-0215(19990315)80:6<857::aid-ijc10>3.0.co;2-b.


We here summarize the analysis of 126 DNA samples from patients with hematopoietic neoplasias and solid tumors and from various tumor cell lines that were screened in the tumorigenicity assay. Thirty-eight samples were able to induce tumors after transfection in NIH/3T3 cells and injection into nude mice. Southern-blot analysis with a panel of oncogene probes revealed human ras genes in the vast majority of cases (25 N-ras, 2 K-ras, 1 H-ras) but also activated FGF4, dbl, ret and mas genes respectively. DNA samples from the 6 remaining transfectants were cloned into EMBL-3 phages and screened with a human specific repetitive Alu probe. Direct hybridization of a transfectant cDNA library allowed cloning of the ufo oncogene. Application of the exon-trapping technique to alu-positive phage DNA from the other transfectants enabled us to isolate tre, cot, B-raf, p85beta/HUMORF8 and a novel oncogene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3 Cells / transplantation
  • Animals
  • Cell Transformation, Neoplastic / genetics*
  • Cloning, Molecular
  • DNA, Neoplasm / genetics*
  • DNA, Neoplasm / isolation & purification
  • Hematologic Neoplasms / genetics
  • Humans
  • Mice
  • Mice, Nude
  • Neoplasm Transplantation
  • Neoplasms / genetics*
  • Oncogenes*
  • Transfection
  • Tumor Cells, Cultured / transplantation


  • DNA, Neoplasm