Characterisation of a Second Patched Gene in the Zebrafish Danio Rerio and the Differential Response of Patched Genes to Hedgehog Signalling

Dev Biol. 1999 Apr 1;208(1):14-29. doi: 10.1006/dbio.1998.9169.


Hedgehog signalling has been implicated in a variety of processes in vertebrate development, and in each case, the activity of Hh proteins is thought to be mediated by their interaction with a large multipass transmembrane protein encoded by the patched (ptc) gene. In this study we present the full-length coding sequence and describe the wild-type expression pattern of a second ptc gene in zebrafish, Zf-ptc2. We find that at the sequence level Zf-ptc2 is more closely related than Zf-ptc1 to the ptc genes initially characterised in other vertebrate species. We also show that transcription of Zf-ptc2, like Zf-ptc1, is dependent upon Hh signalling and present evidence that it is activated in response to lower levels of Hh activity than is Zf-ptc1. In addition we find no evidence for any specificity in the regulatory interactions between the various Hh proteins and the two ptc genes in the zebrafish.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cloning, Molecular
  • Embryonic Development
  • Gene Expression Regulation, Developmental / genetics
  • Hedgehog Proteins
  • Membrane Proteins / chemistry
  • Membrane Proteins / genetics*
  • Microinjections
  • Molecular Sequence Data
  • Mutation
  • Patched Receptors
  • Patched-1 Receptor
  • Phylogeny
  • Proteins / genetics*
  • RNA, Antisense / genetics
  • RNA, Messenger / metabolism
  • Receptors, Cell Surface
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Signal Transduction / genetics
  • Trans-Activators*
  • Transcription, Genetic / genetics
  • Zebrafish / genetics*
  • Zebrafish Proteins*


  • Hedgehog Proteins
  • Membrane Proteins
  • Patched Receptors
  • Patched-1 Receptor
  • Proteins
  • RNA, Antisense
  • RNA, Messenger
  • Receptors, Cell Surface
  • Trans-Activators
  • Zebrafish Proteins
  • ptch1 protein, zebrafish