Tobacco BY-2 cell-free extracts induce the recovery of microtubule nucleating activity of inactivated mammalian centrosomes

Biochim Biophys Acta. 1999 Feb 4;1449(1):101-6. doi: 10.1016/s0167-4889(98)00179-7.


The structure and the molecular composition of the microtubule-organizing centers in acentriolar higher plant cells remain unknown. We developed an in vitro complementation assay where tobacco BY-2 extracts can restore the microtubule-nucleating activity of urea-inactivated mammalian centrosomes. Our results provide first evidence that soluble microtubule-nucleating factors are present in the plant cytosolic fraction. The implication for microtubule nucleation in higher plants is discussed.

MeSH terms

  • Centrosome / chemistry
  • Centrosome / drug effects*
  • Centrosome / physiology
  • Microtubules / physiology
  • Plant Extracts / pharmacology
  • Plants, Toxic*
  • Tobacco / genetics
  • Tobacco / metabolism*
  • Tobacco / ultrastructure
  • Urea


  • Plant Extracts
  • Urea