NMR study of the metabolic 15N isotopic enrichment of cyanophycin synthesized by the cyanobacterium Synechocystis sp. strain PCC 6308

Biochim Biophys Acta. 1999 Feb 2;1426(3):429-38. doi: 10.1016/s0304-4165(98)00164-0.

Abstract

1H, 13C and 15N nuclear magnetic resonance (NMR) spectroscopy has been used to characterize cyanophycin, a multi-l-arginyl-poly-[l-aspartic acid] polypeptide from the cyanobacterium Synechocystis sp. strain PCC 6308. 1H, 13C and 15N chemical shifts and 1JHN and 1JCN coupling constants were measured in isolated 15N-labeled cyanophycin, and showed chemical shift values and J-couplings consistent with the reported polypeptide structure. 15N enrichment levels were determined from the extent of 1H-15N J-coupling in 1H NMR spectra of cyanophycin. Similar experiments using 13C-15N coupling in 13C NMR spectra were not useful in determining enrichment levels.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Bacterial Proteins
  • Cyanobacteria / genetics
  • Cyanobacteria / metabolism*
  • Hydrogen-Ion Concentration
  • Magnetic Resonance Spectroscopy / methods
  • Nitrogen Isotopes
  • Plant Proteins / biosynthesis
  • Plant Proteins / chemistry*
  • Temperature

Substances

  • Bacterial Proteins
  • Nitrogen Isotopes
  • Plant Proteins
  • cyanophycin