NADH reoxidation does not control glycolytic flux during exposure of respiring Saccharomyces cerevisiae cultures to glucose excess

FEMS Microbiol Lett. 1999 Feb 15;171(2):133-40. doi: 10.1111/j.1574-6968.1999.tb13423.x.


Introduction of the Lactobacillus casei lactate dehydrogenase (LDH) gene into Saccharomyces cerevisiae under the control of the TPI1 promoter yielded high LDH levels in batch and chemostat cultures. LDH expression did not affect the dilution rate above which respiro-fermentative metabolism occurred (Dc) in aerobic, glucose-limited chemostats. Above Dc, the LDH-expressing strain produced both ethanol and lactate, but its overall fermentation rate was the same as in wild-type cultures. Exposure of respiring, LDH-expressing cultures to glucose excess triggered simultaneous ethanol and lactate production. However, the specific glucose consumption rate was not affected, indicating that NADH reoxidation does not control glycolytic flux under these conditions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aerobiosis
  • Cloning, Molecular
  • Fermentation / drug effects
  • Gene Expression Regulation, Bacterial / drug effects
  • Gene Expression Regulation, Fungal / drug effects
  • Genes, Bacterial / physiology
  • Glucose / pharmacology*
  • Glycogen / metabolism
  • L-Lactate Dehydrogenase / genetics*
  • L-Lactate Dehydrogenase / metabolism
  • Lactobacillus casei / genetics
  • NAD / metabolism*
  • Oxidation-Reduction
  • Saccharomyces cerevisiae / enzymology*
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / growth & development


  • NAD
  • Glycogen
  • L-Lactate Dehydrogenase
  • Glucose