Changes in caspase expression in Alzheimer's disease: comparison with development and aging

Biochem Biophys Res Commun. 1999 Mar 16;256(2):381-4. doi: 10.1006/bbrc.1999.0344.

Abstract

Changes in caspase-2 and -3 protein levels in Alzheimer's disease (AD) brains were assessed in comparison with their expression in development and aging. The protein levels of caspase-2 and -3 were significantly increased in AD brains, caspase-2 in the particulate fraction and caspase-3 in both the cytosolic and particulate fractions, compared with controls. Immunoblot analysis of brain extracts from embryonic day 19 (E19) to postnatal 96-week-old rats indicated that caspase-3 levels were high from E19 to 2 weeks, while caspase-2 remained high from 4 to 96 weeks, indicating that the expression of caspase-2 and -3 is differentially regulated during development and aging. These results suggest that caspase-2 and -3 are upregulated in AD and that the changes in AD are different from those observed in senescent brains.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Aging / metabolism*
  • Alzheimer Disease / enzymology*
  • Animals
  • Blotting, Western
  • Caspase 2
  • Caspase 3
  • Caspases / genetics
  • Caspases / metabolism*
  • Cerebral Cortex / embryology
  • Cerebral Cortex / enzymology*
  • Cytosol / enzymology
  • Enzyme Precursors / genetics
  • Enzyme Precursors / metabolism
  • Female
  • Gene Expression Regulation, Developmental
  • Gene Expression Regulation, Enzymologic*
  • Humans
  • Male
  • Matched-Pair Analysis
  • Molecular Weight
  • Rats
  • Rats, Wistar
  • Solubility
  • Up-Regulation

Substances

  • Enzyme Precursors
  • CASP3 protein, human
  • Casp3 protein, rat
  • Caspase 2
  • Caspase 3
  • Caspases