Helicobacter pylori in dental plaque: a comparison of different PCR primer sets

Dig Dis Sci. 1999 Mar;44(3):479-84. doi: 10.1023/a:1026680618122.


This study was designed to compare different primer sets for PCR analysis of H. pylori in the same series of 40 dental plaque samples. Three pairs of primers, HPU1/HPU2, HP1/HP2, and EHC-U/EHC-L, directed to the urease A gene, 16S rRNA gene, or 860-bp DNA of H. pylori, respectively, were used. Our results demonstrate that EHC-L/EHC-U were more specific and sensitive for H. pylori added to saliva or dental plaque than HPU1/HPU2 and HP1/HP2. The detection rates for H. pylori DNA in dental plaque samples from randomly selected adult patients from the Dental Clinic of the University of Ulm were 26.5% (9/34) for HPU1/HPU2, 78.9% (30/38) for HP1/HP2, and 100% (40/40) for EHC-U/EHC-L (P < 0.001). Nested PCR using primers directed to the 860-bp DNA of H. pylori further confirmed the presence of H. pylori DNA (40/40) in all these samples. Our results indicate that primers EHC-U/EHC-L are to be recommended for PCR detection of H. pylori in the oral cavity.

Publication types

  • Comparative Study

MeSH terms

  • Adult
  • DNA Primers
  • DNA, Bacterial / isolation & purification
  • Dental Plaque / microbiology*
  • Female
  • Helicobacter pylori / genetics
  • Helicobacter pylori / isolation & purification*
  • Humans
  • Male
  • Polymerase Chain Reaction / methods
  • Random Allocation
  • Saliva / microbiology
  • Sensitivity and Specificity


  • DNA Primers
  • DNA, Bacterial