Impaired binding of a DQ2 and DQ8-binding HSV VP16 peptide to a DQA1*0501/DQB1*0302 trans class II heterodimer

Tissue Antigens. 1999 Jan;53(1):101-5. doi: 10.1034/j.1399-0039.1999.530111.x.

Abstract

DQalpha and DQbeta trans heterodimeric HLA-DQ molecules form in individuals heterozygous for the DQ2 and DQ8 specificities. Unique functions and disease associations have been postulated for such trans-dimers, which may be different from cis-encoded DQ molecules encoded by the corresponding haplotypes. We analyzed the ability of the trans-dimer encoded by HLA-DQA1*0501/DQB1*0302 to bind a peptide antigen which interacts with DQ molecules encoded by both parental haplotypes. Markedly impaired binding was observed, consistent with both the use of different anchor residues and with changes in levels of DQ cis-dimer availability for peptide binding interactions.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology
  • Autoimmune Diseases / genetics
  • Autoimmune Diseases / immunology
  • B-Lymphocytes
  • Cell Line, Transformed
  • Dimerization
  • Genes, MHC Class II
  • Genetic Predisposition to Disease
  • HLA-DQ Antigens / chemistry
  • HLA-DQ Antigens / genetics
  • HLA-DQ Antigens / metabolism*
  • HLA-DQ alpha-Chains
  • HLA-DQ beta-Chains
  • Haplotypes / genetics
  • Herpes Simplex Virus Protein Vmw65 / metabolism*
  • Herpesvirus 4, Human
  • Humans
  • Mice
  • Protein Binding
  • Protein Multimerization
  • Recombinant Fusion Proteins / immunology
  • Recombinant Fusion Proteins / metabolism
  • Severe Combined Immunodeficiency / pathology
  • Transfection

Substances

  • Antibodies, Monoclonal
  • HLA-DQ Antigens
  • HLA-DQ alpha-Chains
  • HLA-DQ beta-Chains
  • HLA-DQ2 antigen
  • HLA-DQ8 antigen
  • HLA-DQA1 antigen
  • HLA-DQB1 antigen
  • Herpes Simplex Virus Protein Vmw65
  • Recombinant Fusion Proteins