Identification and mutation analysis of DOC-1R, a DOC-1 growth suppressor-related gene

Biochem Biophys Res Commun. 1999 Feb 5;255(1):59-63. doi: 10.1006/bbrc.1999.0148.


The tumor suppressor gene MEN1 and several oncogenes including CCND1/cyclin D1/PRAD1 map to chromosome 11q13. However, molecular and cytogenetic analysis suggests the presence of a second tumor suppressor locus at this chromosome region. We have identified a novel gene from chromosome 11q13, which encodes a protein of 126 amino acids sharing an overall 57% identity with the p12(DOC-1) protein encoded by the DOC-1 gene, the human homolog of hamster putative tumor suppressor doc-1 (deleted in oral cancer-1). We therefore designated the novel gene as DOC-1R for DOC-1-related. The cytogenetic location was confirmed by chromosome fluorescent in situ hybridization. Northern blot analysis indicated that it was expressed in all the tissues examined. DOC-1R protein showed heterogeneous subcellular localization. RT-PCR-SSCP analysis failed to detect deleterious mutations of the DOC-1R transcript in four premalignant oral keratinocyte lines and 20 different cancer cell lines from tumor types which frequently harbor LOH at chromosome 11q13.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cell Line
  • Chromosome Mapping
  • Chromosomes, Human, Pair 11*
  • Cricetinae
  • DNA Mutational Analysis
  • Genes, Tumor Suppressor*
  • Humans
  • Molecular Sequence Data
  • Oncogene Proteins / genetics*
  • Proteins / genetics*
  • Sequence Alignment
  • Tumor Suppressor Proteins*


  • CDK2AP1 protein, human
  • CDK2AP2 protein, human
  • Oncogene Proteins
  • Proteins
  • Tumor Suppressor Proteins