Prolactin-releasing peptide-immunoreactivity in A1 and A2 noradrenergic neurons of the rat medulla

Brain Res. 1999 Mar 20;822(1-2):276-9. doi: 10.1016/s0006-8993(99)01153-1.


Distribution of prolactin-releasing peptide-like immunoreactivity (PrRP-LI) was investigated in the rat medulla with the use of a rabbit polyclonal antiserum against the human PrRP-31 peptide. PrRP-positive neurons were noted mainly in two areas of the caudal medulla: ventrolateral reticular formation and commissural nucleus of the nucleus of the solitary tract (NTS), corresponding to the A1 and A2 areas. PrRP-LI neurons were absent in the medulla rostral to the area postrema. Double-labeling the sections with PrRP antisera and tyrosine hydroxylase (TH) monoclonal antibodies revealed extensive colocalization of PrRP- and TH-like immunoreactivity (TH-LI) in neurons of the A1 and A2 areas. Our results show that PrRP-LI is expressed in a population of A1 and A2 noradrenergic neurons of the rat caudal medulla.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Female
  • Hypothalamic Hormones / analysis*
  • Hypothalamic Hormones / immunology
  • Male
  • Neurons / chemistry*
  • Neurons / enzymology
  • Neuropeptides / analysis*
  • Neuropeptides / immunology
  • Norepinephrine / physiology*
  • Prolactin / analysis
  • Prolactin / immunology
  • Prolactin-Releasing Hormone
  • Rats
  • Reticular Formation / chemistry
  • Reticular Formation / cytology
  • Solitary Nucleus / chemistry*
  • Solitary Nucleus / cytology
  • Tyrosine 3-Monooxygenase / analysis


  • Antibodies, Monoclonal
  • Hypothalamic Hormones
  • Neuropeptides
  • PRLH protein, human
  • Prlh protein, rat
  • Prolactin-Releasing Hormone
  • Prolactin
  • Tyrosine 3-Monooxygenase
  • Norepinephrine